Task #12222 (closed)
Opened 10 years ago
Closed 10 years ago
Bug: ND2 line scan Z stack
| Reported by: | wmoore | Owned by: | mlinkert |
|---|---|---|---|
| Priority: | critical | Milestone: | 5.0.2 |
| Component: | Bio-Formats | Version: | n.a. |
| Keywords: | n.a. | Cc: | david.knecht@… |
| Resources: | n.a. | Referenced By: | n.a. |
| References: | n.a. | Remaining Time: | n.a. |
| Sprint: | n.a. |
Description
From ome-users:
I tried to import a Nikon nd2 file today using Bioformats. This normally works, and works for other files, but this data is a line scan z series and somehow the data must be saved in a different way as it does not open correctly in Fiji/ImageJ. The image comes through as a stack of 3 images rather than a single image and the pixel values and locations are wrong. I can send a sample nd2 file.
Uploaded to QA: 9193.
The file looks correct in the sense of being 512x18, but the pixel values are all wrong and the layout is wrong. There should be a gaussian distribution of pixel values centered around a horizontal maximum at about 3000 intensity. Instead, there is a single line of pixel values at 26000.
Change History (1)
comment:1 Changed 10 years ago by mlinkert
- Resolution set to fixed
- Status changed from new to closed
Fixed with https://github.com/openmicroscopy/bioformats/pull/1085. New builds are not yet available, but this should be included in 5.0.2.