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Task #12885 (closed)

Opened 9 years ago

Closed 9 years ago

Bug: CZI BitsPerPixel

Reported by: wmoore Owned by: mlinkert
Priority: critical Milestone: 5.1.3
Component: Bio-Formats Version: n.a.
Keywords: n.a. Cc:
Resources: n.a. Referenced By: n.a.
References: n.a. Remaining Time: n.a.
Sprint: n.a.

Description (last modified by wmoore)

See http://lists.openmicroscopy.org.uk/pipermail/ome-users/2015-May/005379.html

With the czi file (attached) Bio-formats sets Pixels Significant Bits to 16, whereas the image Original Metadata says BitsPerPixel?: 8.

UPDATE: Another ome-users e-mail today:

"Dear Will,
Thanks for your help.
The interesting thing is that when there are several other .czi files under a folder, the error will recur. You can try to open a .czi file after you unzip the attachment, and then you'll get what I find.
I tested several versions of bioformats, and I found that the error exists from version 5.0.7 to 5.1.1. However, version 5.0.6 to 5.1.0-m3 is fine.
I hope the information will make me understood. Thanks again.
"

Attachments (1)

ZEN 2 Blue 8bit.czi (678.7 KB) - added by wmoore 9 years ago.

Download all attachments as: .zip

Change History (4)

Changed 9 years ago by wmoore

comment:1 Changed 9 years ago by wmoore

  • Description modified (diff)

comment:2 Changed 9 years ago by mlinkert

  • Milestone changed from 5.1.2 to 5.1.3

comment:3 Changed 9 years ago by mlinkert

  • Resolution set to worksforme
  • Status changed from new to closed

This all works as expected with current develop. The pixels really are stored as 16-bit, but the higher 8 bits are not used - so SignificantBits? is set to 8. Opening the file in ImageJ shows a single plane, and the image matches what I see in Zen.

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