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- Timestamp:
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12/21/10 16:48:51 (13 years ago)
- Author:
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saloynton
- Comment:
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2010-12-09 (21)
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2010-12-23 (22)
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| 3 | 3 | UPDATED: Thomas's work has been effected by the snow and so I will be contacted the week beginning of the 6th of December for a re-arranged meeting. |
| 4 | 4 | |
| 5 | | UPDATED: Spoke with Thomas on 8th Dec and microscope session arranged for 16th Dec. Awaiting email to confirm. |
| | 5 | UPDATED: Spoke with Thomas on 8th Dec and microscope session arranged for 16th Dec. |
| | 6 | |
| | 7 | UPDATED: Meeting Notes 16th Dec |
| | 8 | |
| | 9 | The steps taken by Thomas in softworks when viewing his images: |
| | 10 | |
| | 11 | Rendered image > quick projection > Image Stitching (if required) > Volume viewer |
| | 12 | |
| | 13 | The type of image Thomas is viewing determines the step of image stitching. The larger germ line images are stitched at this point in the workflow. The images we were viewing for the demo were cells and so were not stitched. The analysis conducted with the volume viewer was for the measurement of signal intensity. (See annotated screenshot for details) |
| | 14 | |
| | 15 | During this process Thomas highlighted the issue of having to manage and deal with the large images require a large amount of his time. From the start of the start of the process of de-convolution to the projection of a germ line every single image that will typically have 90 – 120 stack, in addition to the images are stitched together with several panels. |
| | 16 | |
| | 17 | The zooming process for softworks was performed either with the zoom bar on screen or with the mouse roller wheel. This was comparable to experience of gmaps. (See annotated screenshot 1 for details). The only other notable detail in the viewer was the use of the scale bar at the bottom of the image. |
| | 18 | |
| | 19 | The remaining discussion with Thomas focused upon his analysis work. The analysis Thomas had been currently working with required him marking out points on the images to calculate the average signal intensity. He also needs to be able to go on to calculate the average intensity around the cell membrane without the bias. Thomas mentioned the new version of emaris is able to do this calculation. (See annotated screenshot 2 for details) |
| | 20 | |
| | 21 | Follow up actions: Keep Thomas updated on continuing work scheduled for week beginning 7th Feb 2011 , possibility to get more screenshots if required |
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