| 1 | # |
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| 2 | #Tag 0x00FF0408 |
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| 3 | |
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| 4 | Resolve3D softWoRx-Acquire Version: 4.0.0 Release 16 |
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| 5 | |
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| 6 | Running on host: cx60428 |
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| 7 | |
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| 8 | Resolve3D Image File: /data1/david/tmp//bioformats-test-GFP-cherry_R3D.dv |
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| 9 | |
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| 10 | Created Wed Jun 13 13:46:42 2012 |
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| 11 | |
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| 12 | |
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| 13 | Optics: |
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| 14 | Objective: Olympus 100X/1.40, UPLS Apo, UIS2, 1-U2B836 |
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| 15 | Lens ID: 10007 |
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| 16 | Aux Magn: 1.00 |
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| 17 | |
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| 18 | Image Properties: |
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| 19 | XY Dimensions: 512 x 512 |
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| 20 | ZWT Dimensions (expected): 1 x 2 x 1 |
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| 21 | Pixel Size: 0.12880 0.12880 0.200 |
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| 22 | Binning: 2x2 |
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| 23 | Flat-field Calibration: Off |
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| 24 | |
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| 25 | Camera: |
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| 26 | Type: CoolSNAP_HQ2 / HQ2-ICX285 |
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| 27 | Gain: 1.00X |
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| 28 | Speed: 10000KHz |
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| 29 | Temp Setting: -25.0C |
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| 30 | |
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| 31 | |
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| 32 | Resolve3D Macro: |
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| 33 | -------------------------------------------------------- |
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| 34 | # Created by DeltaVision Experiment Designer (version: 4.0.0 Release 16) |
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| 35 | # Experiment Macro Name: Resolve3D |
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| 36 | |
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| 37 | # Open image file |
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| 38 | OPF |
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| 39 | |
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| 40 | FILTERS GFP,GFP,100% |
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| 41 | ACTSHUT 17 |
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| 42 | CCD 1.000 |
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| 43 | WRT |
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| 44 | FILTERS mCherry,mCherry,100% |
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| 45 | ACTSHUT 17 |
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| 46 | CCD 1.000 |
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| 47 | WRT |
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| 48 | # Close image file(s) |
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| 49 | CLF |
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| 50 | |
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| 51 | BEEP |
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| 52 | |
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| 53 | # |
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| 54 | # |
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| 55 | #KEY Experiment Designer Keys: Don't Modify |
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| 56 | # |
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| 57 | #KEY EXPERIMENT_TYPE STANDARD |
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| 58 | #KEY DO_FAST_ACQUISITION F |
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| 59 | #KEY LAMPS_OFF_AT_END F |
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| 60 | #KEY DO_Z_SECTIONING F |
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| 61 | #KEY CHANNEL 1.0000,GFP,GFP,100% |
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| 62 | #KEY EX_SHUTTERS 4,EX=T,TRANS=F,EX2=F,LASER=F |
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| 63 | #KEY EM_SHUTTERS 1,CCD1=T |
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| 64 | #KEY CHANNEL 1.0000,mCherry,mCherry,100% |
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| 65 | #KEY EX_SHUTTERS 4,EX=T,TRANS=F,EX2=F,LASER=F |
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| 66 | #KEY EM_SHUTTERS 1,CCD1=T |
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| 67 | #KEY DO_REFERENCE_IMAGE F |
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| 68 | #KEY DO_CELL_TRACK F |
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| 69 | #KEY DO_AUTOFOCUS F |
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| 70 | #KEY DO_OAI_SCANNING F |
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| 71 | #KEY DO_TIME_LAPSE F |
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| 72 | #KEY DO_POINT_VISITING F |
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| 73 | #KEY DO_PANEL_COLLECTION F |
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| 74 | #KEY DO_MX_CHANNELS F |
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| 75 | # |
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| 76 | # |
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| 77 | -------------------------------------------------------- |
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| 78 | |
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| 79 | Experiment Record: |
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| 80 | ------------------ |
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| 81 | Image 1. |
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| 82 | Time: Wed Jun 13 13:46:44 2012 |
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| 83 | Time Point: 0.000 secs |
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| 84 | Intensity: Min = 133, Max = 283, Mean = 165.28247 |
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| 85 | Photosensor reading: 144729872.0 |
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| 86 | Exposure time: 1.000 secs |
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| 87 | EX filter: GFP |
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| 88 | EM filter: GFP |
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| 89 | ND filter: 100% |
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| 90 | Stage coordinates: (-2148.680,+86.799,-11.729) |
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| 91 | |
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| 92 | Image 2. |
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| 93 | Time: Wed Jun 13 13:46:45 2012 |
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| 94 | Time Point: 1.387 secs |
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| 95 | Intensity: Min = 99, Max = 202, Mean = 117.71366 |
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| 96 | Photosensor reading: 200574224.0 |
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| 97 | Exposure time: 1.000 secs |
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| 98 | EX filter: mCherry |
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| 99 | EM filter: mCherry |
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| 100 | ND filter: 100% |
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| 101 | Stage coordinates: (-2148.680,+86.799,-11.729) |
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| 102 | |
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| 103 | Total number of images saved: 2 |
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